| Date | Home | Away | Result |
|---|---|---|---|
| 12/09/25 17:30 | Arminia Bielefeld | 1. FC Magdeburg | 2-0 |
| 12/09/25 17:30 | SC Paderborn 07 | VfL Bochum | 1-0 |
| 13/09/25 12:00 | FC Schalke 04 | Holstein Kiel | 0-1 |
| 13/09/25 12:00 | SV Darmstadt 98 | Eintracht Braunschweig | 2-1 |
| 13/09/25 12:00 | Karlsruher SC | 1. FC Nürnberg | 2-1 |
| 13/09/25 19:30 | Hannover 96 | Hertha BSC | 0-3 |
| 14/09/25 12:30 | Preußen Münster | Fortuna Düsseldorf | --- |
| 14/09/25 12:30 | SV Elversberg | Dynamo Dresden | --- |
| 14/09/25 12:30 | SpVgg Greuther Fürth | 1. FC Kaiserslautern | --- |